Novel Coronavirus (SARS-CoV-2) Multiplex Real-Time PCR Pusa

Faʻamatalaga puʻupuʻu:

O le tala fou coronavirus o se RNA siama, lea e aofia ai proteins ma nucleic acids. O le siama e osofaʻia le talimalo (tagata) tino, ulufale i sela e ala i le fusifusia 'upega fesoʻotaʻiga talileleia ACE2, ma faʻapipiʻi i sela talimalo, mafua ai le puipuiga o le tagata tino i tali mai fafo osofaʻia ma maua ai ni vailaʻau faʻapitoa. O le mea lea, o le vial nucleic acid ma antigens, ma vaega faʻapitoa e teteʻe atu ai i tala fou o le coronavirus e mafai ona faʻaaogaina faʻapitoa e avea ma biomarkers faʻapitoa mo le mauaina o le coronavirus fou. Mo le faʻamalamalamaina o le nucleic acid, o le RT-PCR tekonolosi e masani ona faʻaaogaina.

Novel Coronavirus (SARS-CoV-2) Multiplex Real-Time PCR Kit o loʻo fuafua e faʻaaoga e faʻamanuiaina ai le mauaina o le SARS_CoV-2 viral RNA na aumaia mai i nasofaryngeal swabs, oropharyngeal swabs, sputum ma BALF mai tagata mamaʻi e fesoʻotaʻi ma le FDA / CE IVD extraction system ma tulaga filifilia PCR o loʻo lisi atu i luga.

O le pusa e faʻamoemoe e faʻaaoga e le aufaigaluega aʻoaʻoina falesuesue


Oloa Faʻamatalaga

Pine o oloa

Lenei sili atu maaleale, sauni-e-faʻaaoga pusa PCR o loʻo avanoa i le lyophilized faʻataʻitaʻiga (liu aisa-faʻamago gaioiga) mo se taimi umi teuina. O le pusa e mafai ona aveina ma teu i le potu vevela ma e mausali mo le tasi le tausaga. Taʻitasi faagaau o premix aofia uma reagents manaʻomia mo le PCR amplification, aofia ai Reverse-transcriptase, Taq polymerase, primers, probes, ma dNTPs substrates. E naʻo le faʻaopopoina o le 13ul distill vai ma le 5ul aveʻese mai le RNA template, ona mafai lea ona tamoʻe ma faʻateleina i mea fai a le PCR.

O le masini qPCR tatau ona faʻamalieina manaʻoga nei:
1. Fetaui 8 fasimea PCR paipa voluma 0.2 ml
2. Ia sili atu ma le fa auala vaʻaia.

Channel

Fiafia (nm)

Emission (nm)

Lanu Muai Fofoina

1.

470

525

FAM, SYBR Green I

2

523

564

VIC, HEX, TET, JOE

3.

571

621

ROX, TEXAS-Mumu

4

630

670

CY5

PCR-fausaga opea:
7500Real-Time PCR System, Biorad CF96, iCycler iQ ™ Real-Time PCR Detection System, Stratagene Mx3000P, Mx3005P

Le faigata o felauaiga malulu filifili o Novel Coronavirus nucleic acid mauaina mauaina
Pe a masani ona feaveaʻia vaʻaia vaʻavaʻaia nucleic acid i mea mamao, o le (-20 ± 5) ℃ malulu filifili filifili ma felauaʻiga e manaʻomia e faʻamautinoa ai o le bioactive o le enzyme i le reagents tumau malosi. Ina ia mautinoa o le vevela oʻo i le tulaga masani, e tele kilokalama o le aisa mago e manaʻomia mo atigipusa taʻitasi o le nucleic acid suʻega reagent tusa o lalo ifo o le 50g, ae e naʻo le lua pe tolu aso e mafai ai. I luga o le vaʻaiga a le aufaipisinisi faʻataʻitaʻi, o le mamafa moni o reagents na tuʻuina mai e le au gaosi e laititi atu i le 10% (pe sili mamao atu i lo lenei tau) o le container. Ole tele ole mamafa e sau mai le aisa mago, aisa ma pusa foam, o lona uiga e taugata tele le tau o femalagaiga.

I le masina o Mati 2020, na amata ai ona malepe le COVID-19 i se tulaga tele i fafo atu o le atunuʻu, ma o le manaʻoga o le Novel Coronavirus nucleic acid detagent ua faʻateleina tele. E ui lava i le taugata o le auina atu i fafo o le reagents i le malulu filifili, tele o tagata gaosi mafai lava ona taliaina ona o le tele tele ma maualuga tupe mama.

Peitaʻi, i le faʻaleleia atili o tulafono faʻavaomalo mo oloa auina atu i fafo, faʻapea foi ma le faʻaleleia o le pulega a le atunuʻu i luga o le tafe o tagata ma logistics, o loʻo i ai le faʻaopoopoga ma le le mautonu i le taimi o femalagaaiga a aliʻi, na mafua ai faʻafitauli iloga o oloa. e ala i femalagaiga. O le umi o femalagaaiga taimi (femalagaaiga taimi pe a ma le afa masina e matua masani ai) e tau atu ai i le tele o taimi oloa le manuia pe a oʻo le oloa i le tagata o tausia. Lenei ua faʻalavelaveina le tele o nucleic acid faʻalauteleina pisinisi auina atu i fafo.

Lyophilized tekonolosi mo PCR reagent fesoasoani i le felauaiga o Novel Coronavirus nucleic acid mauaina mauaina reagent lalolagi atoa

O le lyophilized PCR reagents mafai ona feaveaʻi ma teuina i le potu vevela, lea e le gata mafai faʻaititia ai le tau o felauaiga, ae faʻapea foi ona aloese ai mai le lelei faʻafitauli mafua mai i auala felauaiga. Ole mea lea, ole faʻamalosi tino ole auala ole auala pito sili lea e fofo ai le faʻafitauli ole felauaiga i fafo.

Lyophilization aofia ai le faʻamaʻaina o se fofo i totonu o se mautu tulaga, ona faʻamamaina ma tuueseese le ausa vai i lalo o lagona gaogao. O le solute solute o loʻo tumau pea i totonu o le koneteina ma le tutusa tuʻufaʻatasiga ma gaioiga. Faʻatusatusa i vailaʻau masani suavai, o le atoa-vaega lyophilized Novel Coronavirus nucleic acid mauaina vailaʻau na faia e Liming Bio ei ai uiga nei:

Matua mausali malosi faʻamautuina: e mafai ma le tu togafitiga i le 56 ℃ mo 60 aso, ma le morphology ma faʻatinoina o le reagent tumau le suia.
Teuina masani o le vevela ma felauaiga: leai se manaʻoga mo malulu filifili, leai se mea e teu ai i le maualalo vevela i luma o le faʻamaufaʻailogaina, faʻamatuʻu atoa le malulu teuina avanoa.
Sauni-e-faʻaaoga: lyophilizing o vaega uma, leai se manaʻoga mo le faʻatulagaina o le tino, aloese mai le leiloa o vaega ma maualuga viscosity pei o le enzyme.
Multiplex sini i le tasi faagaau: o le faʻamoemoe mauaina e aofia ai le coronavirus ORF1ab gene, N gene, S gene e aloese ai mai le siama genovariation. Ina ia mafai ona faʻaititia le sese le lelei, o le tagata RNase P gene e faʻaaogaina e avea ma faʻatonutonu i totonu, ina ia mafai ai ona ausia le falemaʻi manaʻoga mo faʻataʻitaʻiga lelei lelei.


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